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goat polyclonal anti mouse af488  (Thermo Fisher)


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    Structured Review

    Thermo Fisher goat polyclonal anti mouse af488
    Goat Polyclonal Anti Mouse Af488, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+mouse+af488+antibody/CHOLINE+CHLORIDE+1KG+1KG/10__1038_slash_s41467___026___71101___y-279-81-85
    Average 97 stars, based on 1 article reviews
    goat polyclonal anti mouse af488 - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Incubation:

    Article Title: Antibodies to biotin enable large-scale detection of biotinylation sites on proteins
    Article Snippet: APEX2 expression was detected by staining the fixed cells with mouse anti-V5 antibody (Life Technologies, Cat. No. R960–25; 1:1,000) in blocking buffer for 1 h at room temperature. .. Samples were then washed 4 × 5 min with PBS and incubated with goat anti-mouse-AF488 antibody (Thermo Fisher Scientific R37120 ; 1:1,000) in blocking buffer for 1 h at room temperature. ..

    Article Title: RIPK4 function interferes with melanoma cell adhesion and metastasis.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense incubated overnight with mouse antivinculin antibody (V9131; 1 : 400; Sigma-Aldrich, St. Louis, MO, USA), followed by a 2-h incubation with goat anti-mouse AF488-antibody (A11029; 1 : 300), AF568-phalloidin (A12380; 1 : 40) and Hoechst 33258 (H1398; 2 μg mL 1; all Thermo Fisher Scientific), after which aqueous specimens were prepared. .. The samples were imaged using a Leica DMI 6000B microscope equipped with a TIRFM module, an HC PL APO 100×/1.47 OIL objective, a DFC360FX camera, a metal halide illuminator and a laser unit operating at 488 nm (all Leica Microsystems).

    Article Title: Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function
    Article Snippet: At 19 hpi cells were fixed in 4% formaldehyde (Sigma-Aldrich) in PBS (Gibco) for 15 min at RT, washed with PBS, and permeabilized as described above. .. Cells were washed with PBS and incubated with antibody against PRRSV nsp2 (A gift from Ying Fang, Kansas State University, [ ], 1:400) in blocking buffer for 1 h, washed, and incubated with secondary goat anti-mouse AF488 antibody (A11029, 1:100), AF568 phalloidin (A12380, 1:100), and DAPI (1:10,000; all Life Technologies). .. The samples were analyzed using a confocal laser-scanning microscope (Zeiss LSM-710).

    Blocking Assay:

    Article Title: Antibodies to biotin enable large-scale detection of biotinylation sites on proteins
    Article Snippet: APEX2 expression was detected by staining the fixed cells with mouse anti-V5 antibody (Life Technologies, Cat. No. R960–25; 1:1,000) in blocking buffer for 1 h at room temperature. .. Samples were then washed 4 × 5 min with PBS and incubated with goat anti-mouse-AF488 antibody (Thermo Fisher Scientific R37120 ; 1:1,000) in blocking buffer for 1 h at room temperature. ..

    Article Title: Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function
    Article Snippet: At 19 hpi cells were fixed in 4% formaldehyde (Sigma-Aldrich) in PBS (Gibco) for 15 min at RT, washed with PBS, and permeabilized as described above. .. Cells were washed with PBS and incubated with antibody against PRRSV nsp2 (A gift from Ying Fang, Kansas State University, [ ], 1:400) in blocking buffer for 1 h, washed, and incubated with secondary goat anti-mouse AF488 antibody (A11029, 1:100), AF568 phalloidin (A12380, 1:100), and DAPI (1:10,000; all Life Technologies). .. The samples were analyzed using a confocal laser-scanning microscope (Zeiss LSM-710).



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